INHIBITION BY MEPACRINE OF AMYLASE SECRETION FROM INTACT AND PERMEABILIZED RAT PANCREATIC ACINI

被引：17

作者：

GROSFILS, K

GOMEZ, F

DEHAYE, JP

机构：

[1] Department of General and Human Biochemistry, Institute of Pharmacy, Free University of Brussels, B-1050 Brussels, Boulevard du Triomphe

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 184卷 / 01期

关键词：

D O I：

10.1016/0006-291X(92)91208-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In intact rat pancreatic acini, the phospholipase A2 inhibitor mepacrine did not affect basal amylase release but dose-dependently inhibited the carbachol (IC50 65μM) and CCK-8 (IC50 210 μM)-stimulated amylase release. In permeabilized acini, mepacrine shifted the dose-response curve for calcium to the right by a factor 2 and inhibited the release of amylase stimulated by GTPτS. From these results we conclude that carbachol, CCK-8 and GTPτS probably activate a phospholipase A2 closely coupled to exocytosis. © 1992.

引用

页码：408 / 413

页数：6

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