DETERMINATION OF CHLOROPHYLL-A AND PHEOPHYTIN A CONCENTRATIONS IN ACETONE EXTRACTS BY LASER FLUOROMETRY

被引：3

作者：

DEMIDOV, AA

BAULIN, EV

机构：

来源：

APPLIED SPECTROSCOPY | 1995年 / 49卷 / 02期

关键词：

CHLOROPHYLL; PHEOPHYTIN; ACETONE EXTRACT; LASER FLUOROMETER; PHYTOPLANKTON; ZOOPLANKTON;

D O I：

10.1366/0003702953963715

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

We have used the technique of laser fluorometry for chlorophyll a and pheophytin a measurements in 90% acetone/water extract of sea-water phytoplankton. In our method, the acetone Raman signal is used as an internal standard. We have shown that use of the second harmonic of a pulsed Nd:YAG laser (lambda = 532 nm) makes it possible to measure pigment concentrations as low as 10 ng/L. It creates favorable conditions for high-efficiency analysis of the phytoplankton pigments in the oligotrophic areas of the world ocean and for investigations of the feed process of individual zooplankton samples.

引用

页码：200 / 206

页数：7

共 41 条

[1] DETERMINATION OF PHYTOPLANKTON PIGMENTS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].