URIDINE PHOSPHORYLASE FROM RAT BRAIN - CHARACTERISTICS AND DEVELOPMENTAL COURSE

被引：14

作者：

GUROFF, G

RHOADS, CA

机构：

[1] Section on Intermediary Metabolism, Laboratory of Biomedical Sciences, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland

来源：

JOURNAL OF NEUROCHEMISTRY | 1969年 / 16卷 / 11期

关键词：

D O I：

10.1111/j.1471-4159.1969.tb09910.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

—Uridine phosphorylase (uridine: orthophosphate ribosyltransferase; EC 2.4.2.3) from rat brain was purified and its properties were studied. The enzyme resembled preparations made from other mammalian sources. Its pH optimum was between 7·6 and 8·0. An examination of its action on various substrates showed rates of reaction in the order: uridine > deoxyuridine > thymidine > cytidine. The enzyme showed a requirement for phosphate which could also be satisfied by arsenate. The activity of the enzyme was protected from heat inactivation by uridine and by phosphate. In brain and liver the activity of the enzyme increased five‐ to ten‐fold between 10 and 20 days of life. Injections of cortisol or of uridine did not increase the enzymic activity. Copyright © 1969, Wiley Blackwell. All rights reserved

引用

页码：1543 / &

共 11 条

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