ENDONUCLEASE CLEAVAGE MAP OF THE PLASMID PWWO-8, A DERIVATIVE OF THE TOL PLASMID OF PSEUDOMONAS-PUTIDA MT-2

被引:30
作者
DOWNING, RG [1 ]
DUGGLEBY, CJ [1 ]
VILLEMS, R [1 ]
BRODA, P [1 ]
机构
[1] UNIV EDINBURGH,DEPT MOLEC BIOL,EDINBURGH EH9 3JR,MIDLOTHIAN,SCOTLAND
来源
MOLECULAR & GENERAL GENETICS | 1979年 / 168卷 / 01期
关键词
D O I
10.1007/BF00267938
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Cleavage sites on the pWWO-8 plasmid were determined for the restriction endonucleases HindIII and XhoI. Terminal labelling using DNA polymerase I was particularly useful both for the characterisation of the smaller cleavage products and for confirmation of the order of fragments in the intact plasmid. © 1979 Springer-Verlag.
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页码:97 / 99
页数:3
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