QUANTIFICATION OF HEMOGLOBIN BINDING OF 4,4'-METHYLENEBIS(2-CHLOROANILINE)(MOCA) IN RATS

被引:23
作者
SABBIONI, G
NEUMANN, HG
机构
[1] Institut für Pharmakologie und Toxikologie, Universität Würzburg, Würzburg, 8700
关键词
4,4′-Methylenebis(2-chloroaniline); Aromatic amines; Biomonitoring; Haemoglobin; Protein adducts;
D O I
10.1007/BF01977626
中图分类号
R99 [毒物学(毒理学)];
学科分类号
100405 ;
摘要
4,4 ′-Methylenebis(2-chloroaniline) (MOCA) is used as a curing agent in the production of polyurethane. MOCA is carcinogenic in experimental animals. Haemoglobin adducts have been proposed as dosimeters of aromatic amines for biological monitoring. A quantitative method to determine the adduct has now been worked out in female Wistar rats dosed per os with 3.82, 14.2 and 16.2 μmol/kg14C-ring labeled MOCA or 0.25 and 0.50 mmol/kg unlabeled MOCA. MOCA bound in decreasing amounts to DNA, RNA and proteins of the lung, liver and kidney. Fractions of 0.19% and 0.026% of the dose were bound to the blood proteins haemoglobin and albumin, respectively. MOCA released by hydrolysis from haemoglobin was determined by HPLC with electrochemical detection or by GC-MS. Albumin did not form any hydrolysable adducts with MOCA. © 1990 Springer-Verlag.
引用
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页码:451 / 458
页数:8
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