STATISTICAL EVALUATION OF THE BINDING OF FERRITIN-CONJUGATED LECTINS TO THE SURFACE OF RAT-CELLS - TOPOGRAPHICAL VARIATIONS AS A FUNCTION OF LECTIN CONCENTRATION AND CELL TYPE

被引:6
作者
HIXSON, DC
MILLER, MF
MARUYAMA, K
WALBORG, EF
WAGNER, S
STARLING, JJ
BOWEN, JM
机构
[1] UNIV TEXAS, MD ANDERSON HOSP & TUMOR INST, DEPT BIOCHEM, HOUSTON, TX 77030 USA
[2] UNIV TEXAS, MD ANDERSON HOSP & TUMOR INST, DEPT MOLEC CARCINOGENESIS, HOUSTON, TX 77030 USA
[3] UNIV TEXAS, MD ANDERSON HOSP & TUMOR INST, DEPT VIROL, HOUSTON, TX 77030 USA
关键词
D O I
10.1177/27.12.316439
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Statistical procedures were utilized to evaluate the concentration dependence of labeling by ferritin-conjugated lectins on four different rat cells: hepatocytes, normal thymocytes, Friend virus-induced rat tumor cells and feline sarcoma virus-induced rat sarcoma cells. Labeling by ferritin conjugates of concanavalin A, wheat germ agglutinin and Ricinus communis agglutinins I and II was quantitated by counting the number of ferritin granules on 600 Å membrane segments. Relationships between the arithmetic means and variances for sample populations from each cell and ferritin-lectin combination were used to define four types of topographical distributions: uniform/ordered, uniform/random, random and clustered. It was found that the distribution and/or density of surface-bound lectin was concentration-dependent for all four ferritin-lectins. The nature of this dependency was complex and varied with both lectin and cell type.
引用
收藏
页码:1618 / 1629
页数:12
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