CAPILLARY ELECTROPHORESIS OF PEPTIDES - ANALYSIS OF ADRENOCORTICOTROPIC HORMONE-RELATED FRAGMENTS

被引：22

作者：

VANDEGOOR, TAAM ^{[1
]}

JANSSEN, PSL ^{[1
]}

VANNISPEN, JW ^{[1
]}

VANZEELAND, MJM ^{[1
]}

EVERAERTS, FM ^{[1
]}

机构：

[1] ORGANON INT BV, SCI DEV GRP, 5340 BH OSS, NETHERLANDS

来源：

JOURNAL OF CHROMATOGRAPHY | 1991年 / 545卷 / 02期

关键词：

D O I：

10.1016/S0021-9673(01)88730-6

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Capillary electrophoresis can be used successfully to analyse small peptides to give additional information to that obtained using high-performance liquid chromatography (HPLC). The separation of a modified adrenocorticotropic hormone (4-9) fragment (Org 2766) and several of its fragments was investigated using capillary zone electrophoresis. Prediction of migration in aqueous systems using pK(a)-related data and the migration behaviour using sodium dodecyl sulphate in the buffer are discussed, as is the choice of buffer systems. The electrophoretic patterns are compared with the HPLC separation.

引用

页码：379 / 389

页数：11

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