Photoaffinity Heterobifunctional Cross-Linking Reagents Based on Azide-Substituted Salicylates

被引：3

作者：

Imai, Nobuyuki ^{[2
]}

Dwyer, Lori D. ^{[1
]}

Kometani, Tadashi ^{[2
]}

Ji, Tae ^{[3
]}

Vanaman, Thomas C. ^{[1
]}

Watt, David S. ^{[2
]}

机构：

[1] Univ Kentucky, Dept Biochem, Lexington, KY 40506 USA

[2] Univ Kentucky, Dept Chem, Lexington, KY 40506 USA

[3] Univ Wyoming, Dept Biochem, Laramie, WY 82071 USA

来源：

BIOCONJUGATE CHEMISTRY | 1990年 / 1卷 / 02期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1021/bc00002a009

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Photoaffinity heterobifunctional cross-linking reagents are described that incorporate a 4-azidosalicylate group at one terminus and an N-hydroxysuccinimidyl ester at the other terminus with a "linking arm" of variable length separating the photoactive and electrophilic termini. Exposure of calmodulin (CaM) to succinimidyl N- [2-[(4-azidosalicyloyl)oxy]ethyl]suberamate (4C) led to monoadducts at Lys-21, Lys-75, and Lys-94. Separation of the monoadducts from CaM and polyadducts, radioiodination, and photolysis in the presence of human erythrocyte plasma membrane Ca2+, Mg2+-ATPase led to calcium-dependent cross-linking with 8% cross-linking efficiency.

引用

页码：144 / 148

页数：5

共 18 条

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