GENOME FINGERPRINTING OF SALMONELLA-TYPHI BY PULSED-FIELD GEL-ELECTROPHORESIS FOR SUBTYPING COMMON PHAGE TYPES

被引:39
作者
NAIR, S
POH, CL
LIM, YS
TAY, L
GOH, KT
机构
[1] NATL UNIV SINGAPORE,FAC MED,DEPT MICROBIOL,SINGAPORE 0511,SINGAPORE
[2] SINGAPORE GEN HOSP,DEPT PATHOL,ENTER BACTERIOL LAB,SINGAPORE 0316,SINGAPORE
[3] MINIST ENVIRONM,DEPT QUARANTINE & EPIDEMIOL,SINGAPORE,SINGAPORE
关键词
D O I
10.1017/S0950268800068400
中图分类号
R1 [预防医学、卫生学];
学科分类号
1004 ; 120402 ;
摘要
The genomic DNA of 39 strains of Salmonella ty phi isolated from local residents and patients who had visited countries in the Asian region was analysed for restriction fragment length polymorphisms (RFLP). Pulsed-field gel electrophoretic (PFGE) analysis of Xba I- and Spe I-generated genomic restriction fragments established 22 PFGE types whereas phage typing differentiated the 39 isolates into 9 distinct phage types. This study showed that PFGE is more discriminatory than phage typing as it is capable of subtyping S. typhi strains of the same phage types. Genetic relatedness among the isolates was determined. Seven major clusters were identified at S-ABS of > 0.80 and the remaining 13 isolates were distributed into minor clusters which were related at S-ABS of less than 0.80. In conclusion, PFGE analysis in conjunction with distance matrix analysis served as a useful tool for delimeating common S. typhi phage types of diverse origins from different geographical locales and separated in time.
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页码:391 / 402
页数:12
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