AN EFFICIENTLY EXPRESSED 5.8S RIBOSOMAL-RNA TAG FOR IN-VIVO STUDIES OF YEAST RIBOSOMAL-RNA BIOSYNTHESIS AND FUNCTION

被引：7

作者：

ABOUELELA, S ^{[1
]}

GOOD, L ^{[1
]}

NAZAR, RN ^{[1
]}

机构：

[1] UNIV GUELPH,DEPT MOLEC BIOL & GENET,GUELPH,ON N1G 2W1,CANADA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1995年 / 1262卷 / 2-3期

基金：

加拿大自然科学与工程研究理事会;

关键词：

TARGETED MUTAGENESIS; RDNA; 5.8S RIBOSOMAL-RNA; INTERNAL TRANSCRIBED SPACER;

D O I：

10.1016/0167-4781(95)00074-Q

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Inefficient expression or detrimental markers have limited mutational analyses of eukaryotic 5.88 rRNA and the associated rDNA transcribed spacers. We have found a neutral, 4-base insertion mutation that effectively tags the 5.8S rRNA for improved studies of rRNA expression, processing and function. Cells expressing the tagged rBNA plasmid contain 50-60% mutant 5.8S rRNA, but show a normal growth rate and polysomal profile and a constant distribution of tagged 5.8S rRNA. The high level of expression also demonstrates that plasmid-associated rDNA is preferentially transcribed over chromosomal copies.

引用

页码：164 / 167

页数：4

共 13 条

[1] Musters, Venema, Van der Linden, Van Heerikhuizen, Klootwijk, Planta, Mol. Cell. Biol., 9, pp. 551-559, (1989)
[2] Chernoff, Vincent, Liebman, EMBO J., 13, pp. 906-913, (1994)
[3] Nogi, Yano, Nomura, Proc. Nad. Acad. Sci. USA, 88, pp. 3962-3966, (1991)
[4] Henry, Wood, Morrissey, Petfalski, Kearsey, Tollervey, EMBO J., 13, pp. 2452-2463, (1994)
[5] Van Ryk, Lee, Nazar, J. Biol. Chem., 265, pp. 8377-8381, (1990)
[6] Abou Elela, Good, Melenkhovets, Nazar, Inhibition of protein synthesis by an efficiently expressed mutation in the yeast 5.8S ribosomal RNA, Nucleic Acids Research, 22, pp. 686-693, (1994)
[7] Good, Abou Elela, Nazar, Tetrahymena ribozyme disrupts rRNA processing in yeast., J Biol Chem, 269, pp. 22169-22172, (1994)
[8] Melenkhovets, Good, Abou Elela, Nazar, J. Mol. Biol., 239, pp. 170-180, (1994)
[9] Nazar, Cell Nucleus, 9, pp. 1-28, (1982)
[10] Good, Nazar, Nucleic Acids Res., 20, (1992)

← 1 2 →