RETINOID INDUCED CHANGES IN CAMP-DEPENDENT PROTEIN-KINASE ACTIVITY DETECTED BY A NEW MINIGEL ASSAY

被引：4

作者：

HOHMANN, P ^{[1
]}

GREENE, RS ^{[1
]}

机构：

[1] NIAGARA UNIV,DEPT BIOL,NIAGARA,NY 14109

来源：

FEBS LETTERS | 1990年 / 261卷 / 01期

关键词：

Phastgel; Protein kinase; cAMP-dependent; Retinoid;

D O I：

10.1016/0014-5793(90)80641-U

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new method for the resolution of protein kinases in nondenaturing minigels was used to analyze the type of PKA induced by retinyl acetate in transformed mouse 10T 1 2 cells. Protein kinases in 0.5 μl aliquots of cell extracts were resolved on nondenaturing gradient Phastgels and assayed for PKA activity in situ using a specific peptide substrate and [32P]ATP. Retinyl acetate caused a decrease in type I and an increase in type II PKA isoforms after about 36 h in culture. The system provides a quick and sensitive means for analyzing the activity of PKA isoforms from small numbers of cells. © 1990.

引用

页码：81 / 84

页数：4

共 14 条

[1]

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[2] CYCLIC AMP-DEPENDENT AND AMP-INDEPENDENT PROTEIN-KINASES AND PROTEIN-PHOSPHORYLATION IN HUMAN PROMYELOCYTIC LEUKEMIA (HL60) CELLS INDUCED TO DIFFERENTIATE BY RETINOIC ACID [J].