SEPARATION OF THE CELLULOLYTIC AND XYLANOLYTIC ENZYMES OF CLOSTRIDIUM-STERCORARIUM

Exoenzymes of the thermophilic, anaerobic bacterium Clostridium stercorarium involved in cellulose and arabinoxylan degradation were resolved by a combination of fast protein liquid chromatographic (FPLC) chromatofocusing and FPLC anion-exchange chromatography. The purified enzymes include an endo-β-1,4-glucanase (Avicelase I), an exo-β-1,4-glucanase (Avicelase II), a β-d-glucosidase, a β-d-xylosidase, an α-l-arabinofuranosidase and multiple xylanases. Two celloxylanases capable of hydrolyzing both cellodextrins and xylans were also purified. © 1990.