SIMPLE MODIFICATION TO AN ENZYMIC METHOD FOR THE PREPARATION OF [GAMMA-P-32]ATP FREE OF SALT AND BUFFER

被引：8

作者：

ENGLAND, PJ

机构：

[1] Department of Biochemistry, Medical School, University ofBristol, Bristol, BS8 ITD England, University Walk

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 93卷 / 02期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1016/S0003-2697(79)80151-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An existing enzymic method for preparing [γ-32P]ATP from 32Pi has been modified toyield [γ-32P]ATP free of salt and buffer. 32P is incorporated into the γ-position of ATP by isotopic exchange in the presence of glyceraldehyde 3-phosphate dehydrogenase and 3-phosphoglycerate kinase. Unreacted 32Pi is separated from [γ-32P]ATP by column chromatography on Dowex 1 bicarbonate. [γ-32P]ATP is eluted with 2 m triethylammonium bicarbonate, which is then completely removed by freeze-drying. © 1979 Academic Press, Inc.

引用

页码：272 / 274

页数：3