FURTHER ASPECTS OF MICROGRANULOCYTOTOXICITY

被引：7

作者：

DREW, SI ^{[1
]}

CARTER, BM ^{[1
]}

GUIDERA, D ^{[1
]}

LEE, KE ^{[1
]}

SASAKI, M ^{[1
]}

TERASAKI, PI ^{[1
]}

GALE, RP ^{[1
]}

机构：

[1] UNIV CALIF LOS ANGELES,SCH MED,DEPT MED,LOS ANGELES,CA 90024

来源：

TRANSFUSION | 1979年 / 19卷 / 04期

关键词：

D O I：

10.1046/j.1537-2995.1979.19479250181.x

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Some aspects influencing the serologic outcome of complement‐dependent granulocyte cytotoxicity are presented. Ficoll‐Hypaque (density 1.060) gradient centrif‐ugation with hypotonic lysis of red blood cells yielded populations of granulocytes with greater than 90 per cent purity. Granulocytes exposed to papain (0.01%) at 24 C for 12 minutes showed enhanced serologic reactivity compared with untreated cells. In addition, enhanced cytotoxicity was promoted by 5 C (cold) as opposed to 22 C (warm) incubation of granulocytes and antibody in the first stage of the microcytotoxicity assay and emphasizes the temperature dependence of the serologic reactions. Neutrophil‐specific leukoagglutinins were not cytotoxic under optimal in vitro conditions. Conversely, a significant proportion of antibodies selected for cytotoxicity failed to agglutinate granulocytes. Cold reactive granulo‐cytotoxins are complement dependent, IgM in nature, and 2‐mercaptoethanol sensitive and do not appear to be immune complexes. By optimizing reaction conditions, granulocyte microcytotoxicity is a valuable addition to in vitro assays detecting immune sensitization against granulocyte surface antigens. 1979 AABB

引用

页码：434 / 443

页数：10

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