STABILIZATION OF CLATHRIN COATED VESICLES BY AMANTADINE, TROMANTADINE AND OTHER HYDROPHOBIC AMINES

被引：62

作者：

PHONPHOK, Y

ROSENTHAL, KS

机构：

[1] NORTHEASTERN OHIO UNIV, COLL MED, DEPT MICROBIOL & IMMUNOL, ROOTSTOWN, OH 44272 USA

[2] KENT STATE UNIV, SCH BIOMED SCI, KENT, OH 44242 USA

来源：

FEBS LETTERS | 1991年 / 281卷 / 1-2期

关键词：

CLATHRIN; CLATHRIN COATED VESICLE; AMANTADINE; TROMANTADINE; HYDROPHOBIC AMINE;

D O I：

10.1016/0014-5793(91)80390-O

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Amantadine and related compounds stabilized the structure of purified pig brain clathrin coated vesicles (CCV) at biologically relevant concentrations. Incubation of purified CCV for 30 min at 25-degrees-C or 37-degrees-C caused the release of clathrin, as determined by a centrifugation assay, and a reduction in the number of coated vesicles, by electron microscopy. Amantadine (10 mM), tromantadine (1 mM), amidine D295 (cyclohexylcarboximidamide-(N-benzyl)hydrochloride) (10 mM), chloroquin (0.1 mM) and monodansylcadaverine (10 mM) significantly reduced the extent of dissociation.

引用

页码：188 / 190

页数：3

共 21 条

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