FAST-ATOM-BOMBARDMENT MASS-SPECTROMETRY COUPLED TO PLANAR GEL-ELECTROPHORESIS

被引：2

作者：

BROWN, SM

BUSCH, KL

机构：

[1] School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta

来源：

SPECTROSCOPY LETTERS | 1991年 / 24卷 / 10期

关键词：

FAB-MS; ELECTROPHORESIS; INTERFACE; PEPTIDES;

D O I：

10.1080/00387019108021761

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

An interface system between planar gel electrophoresis and mass spectrometry is described that includes a means of rapid electrophoretic gel disruption, concentration of extracted sample in a pre-column, and transfer of the sample molecules to the continuous flow FAB source of a mass spectrometer. Spatial resolution achieved on the surface of the gel is on the order of 2 mm diameter for a spot, and 4 mm2 for a band. Sensitivities to 50 picomoles have been demonstrated for the analysis of small peptides with continuous-flow fast atom bombardment mass spectrometry.

引用

页码：1275 / 1286

页数：12

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