GUANOSINE METABOLISM IN ADULT-RAT CARDIAC MYOCYTES - INHIBITION BY ACYCLOVIR AND ANALYSIS OF A METABOLIC PATHWAY

被引:3
作者
GEISBUHLER, TP [1 ]
SCHWAGER, TL [1 ]
ROVETTO, MJ [1 ]
机构
[1] UNIV MISSOURI, DEPT PHYSIOL, COLUMBIA, MO 65212 USA
关键词
CARDIAC MYOCYTES; GUANOSINE; RIBOSE; NUCLEOSIDE METABOLISM;
D O I
10.1016/0022-2828(92)93382-T
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
The metabolic fate of transported guanosine was examined in adult rat cardiac myocytes. Freshly isolated cells were incubated with 50 μm 8-[3H]-guanosine and the purine nucleoside phosphorylase (PNP) inhibitor acyclovir, and the nucleotide products extracted and examined for radiolabel distribution. Acyclovir inhibited guanosine incorporation into the 5′-nucleotide pool up to 66%. The drug did not inhibit guanosine transport. Other experiments using 5′-[3H]-guanosine and 8-[14C]-guanosine in concert as metabolic tracers showed both tritium and radiocarbon in the guanine nucleotide products. We concluded from this study that both a kinase (probably adenosine kinase) and the enzyme pair purine nucleoside phosphorylase/hypoxanthine-guanine phosphoryibosyltransferase are responsible for guanosine salvage in heart cells. © 1992.
引用
收藏
页码:683 / 690
页数:8
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