INVITRO MUTAGENESIS OF A XYLANASE FROM THE EXTREME THERMOPHILE CALDOCELLUM-SACCHAROLYTICUM

被引：1

作者：

LUTHI, E ^{[1
]}

REIF, K ^{[1
]}

JASMAT, NB ^{[1
]}

BERGQUIST, PL ^{[1
]}

机构：

[1] UNIV AUCKLAND,DEPT CELLULAR & MOLEC BIOL,CTR GENE TECHNOL,AUCKLAND,NEW ZEALAND

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 1992年 / 36卷 / 04期

关键词：

D O I：

暂无

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Six mutant xylanases were obtained by in vitro mutagenesis of a xylanase gene from the extremely thermophilic bacterium Caldocellum saccharolyticum. The temperature stability of all enzymes was affected by mutation to various degrees and one of the xylanases had an altered temperature optimum. The mutations had no effect on the pH optimum. The C. saccharolyticum xylanase showed strong homology to several thermophilic and mesophilic xylanases, and comparison of primary sequences allowed the localization of probable active sites and residues involved in thermostability.

引用

页码：503 / 506

页数：4

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