BETA CELLS ARISE FROM GLUCOSE-TRANSPORTER TYPE-2 (GLUT2)-EXPRESSING EPITHELIAL-CELLS OF THE DEVELOPING RAT PANCREAS

被引:120
作者
PANG, K [1 ]
MUKONOWESHURO, C [1 ]
WONG, GG [1 ]
机构
[1] GENET INST INC, EMBRYON GROWTH & REGULATORY PROT GRP, CAMBRIDGE, MA 02140 USA
关键词
INSULIN;
D O I
10.1073/pnas.91.20.9559
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
We observe Glut2 protein in day 11 (E11) rat embryos in an erndodesmal domain containing the pancreatic primordium. Glut2 expression continues as the endodermal epithelium evaginates into the surrounding mesenchyme to form the pancreatic buds. Cells of the dorsal and ventral pancreatic buds maintain Glut2 expression as the epithelium grows and branches to form ducts. As acini form at the ends of the ducts, acinar cells cease Glut2 expression. Insulin protein is first detected in small clusters at the interface between pancreatic epithelium and mesenchyme. These clusters disperse into the interstitial tissue between E13 and E17. At E17 a distinct, larger population of insulin-expressing cells arises in the Glut2-expressing ductal network. Insulin- and Glut2-coexpressing cells then appear to segregate into large aggregates to form the beta cells of the islets of Langerhans. These observations support the hypothesis that two biologically distinct populations of insulin-expressing cells arise during pancreas formation.
引用
收藏
页码:9559 / 9563
页数:5
相关论文
共 25 条
  • [1] HYBRID INSULIN GENES REVEAL A DEVELOPMENTAL LINEAGE FOR PANCREATIC ENDOCRINE-CELLS AND IMPLY A RELATIONSHIP WITH NEURONS
    ALPERT, S
    HANAHAN, D
    TEITELMAN, G
    [J]. CELL, 1988, 53 (02) : 295 - 308
  • [2] ANDREW A, 1976, J EMBRYOL EXP MORPH, V35, P577
  • [3] THE MIDGESTATIONAL HUMAN FETAL PANCREAS CONTAINS CELLS COEXPRESSING ISLET HORMONES
    DEKRIJGER, RR
    AANSTOOT, HJ
    KRANENBURG, G
    REINHARD, M
    VISSER, WJ
    BRUINING, GJ
    [J]. DEVELOPMENTAL BIOLOGY, 1992, 153 (02) : 368 - 375
  • [4] DIFFERENTIAL EXPRESSION OF THE 2 NONALLELIC PROINSULIN GENES IN THE DEVELOPING MOUSE EMBRYO
    DELTOUR, L
    LEDUQUE, P
    BLUME, N
    MADSEN, O
    DUBOIS, P
    JAMI, J
    BUCCHINI, D
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (02) : 527 - 531
  • [5] FONTAINE J, 1977, J EMBRYOL EXP MORPH, V41, P209
  • [6] DO NEURAL CREST CELLS IN THE PANCREAS DIFFERENTIATE INTO SOMATOSTATIN-CONTAINING CELLS
    FONTAINEPERUS, J
    LELIEVRE, C
    DUBOIS, MP
    [J]. CELL AND TISSUE RESEARCH, 1980, 213 (02) : 293 - 299
  • [7] ONSET OF CELL-SPECIFIC GENE-EXPRESSION IN THE DEVELOPING MOUSE PANCREAS
    GITTES, GK
    RUTTER, WJ
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (03) : 1128 - 1132
  • [8] EPITHELIOMESENCHYMAL INTERACTION IN PANCREATIC MORPHOGENESIS
    GOLOSOW, N
    GROBSTEIN, C
    [J]. DEVELOPMENTAL BIOLOGY, 1962, 4 (02) : 242 - &
  • [9] HERRERA PL, 1991, DEVELOPMENT, V113, P1257
  • [10] ON THE ORIGIN OF PANCREATIC ENDOCRINE-CELLS
    LEDOUARIN, NM
    [J]. CELL, 1988, 53 (02) : 169 - 171