RAPID PROTON RELEASE DURING FLASH-INDUCED OXIDATION OF CYTOCHROME-C OXIDASE

被引：28

作者：

NILSSON, T ^{[1
]}

HALLEN, S ^{[1
]}

OLIVEBERG, M ^{[1
]}

机构：

[1] GOTHENBURG UNIV,S-41296 GOTHENBURG,SWEDEN

来源：

FEBS LETTERS | 1990年 / 260卷 / 01期

关键词：

Cytochrome oxidase; Flow-flash; Proton pumping; Vesicle;

D O I：

10.1016/0014-5793(90)80062-N

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Protolytic reactions during the reaction between fully reduced cytochrome oxidase and oxygen have been investigated using the flow-flash methodology. Enzyme reconstituted into phospholipid vesicles has been used and pH changes in the extravesicular medium have been detected with the pH indicator Phenol red. The results show the release of protons to the medium with a rate of 9.102 s-1 during reoxidation. The amplitude of the absorption change corresponds to at least one proton per functional unit of cytochrome oxidase. © 1990.

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页码：45 / 47

页数：3

相关论文

共 12 条

[1]

GIBSON Q, 1967, J BIOL CHEM, V242, P1782

[2] INTERMEDIATE STEPS IN THE REACTION OF CYTOCHROME-OXIDASE WITH MOLECULAR-OXYGEN [J].

HILL, BC ;

GREENWOOD, C ;

NICHOLLS, P .

BIOCHIMICA ET BIOPHYSICA ACTA, 1986, 853 (02) :91-113

[3] PROTON CONCENTRATION JUMPS AND GENERATION OF TRANSMEMBRANE PH-GRADIENTS BY PHOTOLYSIS OF 4-FORMYL-6-METHOXY-3-NITROPHENOXYACETIC ACID [J].

JANKO, K ;

REICHERT, J .

BIOCHIMICA ET BIOPHYSICA ACTA, 1987, 905 (02) :409-416

[4] ELECTROGENIC PROTON-EXCHANGE BETWEEN CYTOCHROME-A3 ACTIVE-CENTER AND M-AQUEOUS PHASE - EVIDENCE FOR CYTOCHROME-A3-ASSOCIATED INPUT PROTON WELL [J].

KONSTANTINOV, A ;

VYGODINA, T ;

ANDREEV, IM .

FEBS LETTERS, 1986, 202 (02) :229-234

[5] TEMPERATURE JUMP AS A NEW TECHNIQUE TO STUDY THE KINETICS OF FAST TRANSPORT OF PROTONS ACROSS MEMBRANES [J].

KRISHNAMOORTHY, G .

BIOCHEMISTRY, 1986, 25 (21) :6666-6671

[6] INTRINSIC UNCOUPLING IN PROTON-PUMPING CYTOCHROME-C OXIDASE - PH-DEPENDENCE OF CYTOCHROME-C OXIDATION IN COUPLED AND UNCOUPLED PHOSPHOLIPID-VESICLES [J].

MAISONPETERI, B ;

MALMSTROM, BG .

BIOCHEMISTRY, 1989, 28 (08) :3156-3160

[7]

MALSTROM BG, 1989, FEBS LETT, V250, P9

[8]

OLIVEBERG M, 1989, IN PRESS BIOCH BIOPH

[9] KINETICS OF REDOX-LINKED PROTON PUMPING ACTIVITY OF NATIVE AND SUBUNIT-III-DEPLETED CYTOCHROME-C OXIDASE - A STOPPED-FLOW INVESTIGATION [J].

SARTI, P ;

JONES, MG ;

ANTONINI, G ;

MALATESTA, F ;

COLOSIMO, A ;

WILSON, MT ;

BRUNORI, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (15) :4876-4880

[10]

THORNSTROM PE, 1984, CHEM SCRIPTA, V24, P230