SPECTROSCOPIC DETECTION OF LIPID-PEROXIDATION PRODUCTS AND STRUCTURAL-CHANGES IN A SPHINGOMYELIN MODEL SYSTEM

被引：36

作者：

LAMBA, OP

LAL, S

YAPPERT, MC

LOU, MF

BORCHMAN, D

机构：

[1] UNIV LOUISVILLE,SCH MED,KENTUCKY LIONS EYE RES INST,DEPT OPHTHALMOL & VISUAL SCI,LOUISVILLE,KY 40292

[2] ALCON LABS INC,FT WORTH,TX 76101

[3] UNIV LOUISVILLE,DEPT CHEM,LOUISVILLE,KY 40292

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1991年 / 1081卷 / 02期

关键词：

LIPID OXIDATION; SPHINGOMYELIN; TERT-BUTYL HYDROPEROXIDE; LIPID BILAYER; CONJUGATED DIENE; NMR; INFRARED; FLUORESCENCE;

D O I：

10.1016/0005-2760(91)90024-C

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The peroxidation induced by tert-butyl hydroperoxide in sphingomyelin from bovine brain was investigated in detail. The lipid peroxidation products resulting from oxidation of lipid acyl chains were detected, identified and characterized by optical absorption. Fourier transform infrared, fluorescence and NMR spectroscopies. The extent of hydrocarbon chain degradation in vitro was quantified by measuring the relative change in absorbance of the peak at 241 nm characteristic of conjugated double bond or diene absorption band. FTIR data revealed that the lipid peroxidation of sphingomyelin disrupted the acyl chain and head group regions resulting in derangement of the ordered membrane.

引用

页码：181 / 187

页数：7

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