IDENTIFICATION OF LARVAL CROSS-REACTIVE AND EGG-SPECIFIC ANTIGENS INVOLVED IN GRANULOMA-FORMATION IN MURINE SCHISTOSOMIASIS-MANSONI

被引:27
作者
LUKACS, NW [1 ]
BOROS, DL [1 ]
机构
[1] WAYNE STATE UNIV,SCH MED,DEPT IMMUNOL & MICROBIOL,DETROIT,MI 48201
关键词
D O I
10.1128/IAI.59.9.3237-3242.1991
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Cross-reactive humoral immune responses between antigens of different developmental stages of the worm Schistosoma mansoni have previously been demonstrated. In contrast, information on antigenic cross-reactivity at the T-cell level is still very sparse. The present study examined the cross-reactive T-cell responses to eggs and crude and fractionated soluble egg antigens (SEA) in infected mice prior to (from 0 to 4 weeks of infection) and after (5 weeks and onwards) egg deposition. Splenic lymphocyte proliferation to unfractionated SEA was detected as early as 2 weeks postinfection and increased rapidly by 4 weeks postinfection. Injections of live eggs into the lungs of infected mice at 4 weeks postinfection demonstrated enhanced granuloma formation, indicating the presence of primed T cells that respond to egg antigens. Further experiments with the artificial granuloma model and polyacrylamide gel electrophoresis-separated SEA fractions demonstrated that in mice infected for 4 weeks the 60- to 66-, 93- to 125-, and > 200-kDa SEA fraction-coated beads elicited significant pulmonary granulomas. By 6 weeks postinfection, when eggs are deposited in the livers, in addition to the cross-reactive fractions (60 to 66, 93 to 125, and > 200 kDa), beads coated with fractions of 25 to 30, 32 to 38, and 70 to 90 kDa also elicited significant granulomatous reactions. These antigenic fractions are considered to have elicited egg stage-specific T-cell responsiveness. In addition hepatic granuloma T cells from the 6th week of infection demonstrated the strongest blastogenic response to the 60- to 66-kDa cross-reactive fraction. Thus, in vitro and in vivo experiments demonstrated T-cell cross-reactivity between the larval and egg stages of the worm. On the basis of these observations, the appearance of the primary circumovum granulomatous response in infected mice is considered to represent the sum of larval cross-reactive and egg-specific T-cell responsiveness.
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页码:3237 / 3242
页数:6
相关论文
共 31 条
[1]  
ABE T, 1984, J IMMUNOL, V132, P2084
[2]   STUDIES ON THE DEVELOPMENT OF ANTI-SCHISTOSOMULAR SURFACE ANTIBODIES BY MICE EXPOSED TO IRRADIATED CERCARIAE, ADULTS AND/OR EGGS OF S-MANSONI [J].
BICKLE, QD ;
FORD, MJ ;
ANDREWS, BJ .
PARASITE IMMUNOLOGY, 1983, 5 (05) :499-511
[3]   IMMUNOPATHOLOGY OF SCHISTOSOMA-MANSONI INFECTION [J].
BOROS, DL .
CLINICAL MICROBIOLOGY REVIEWS, 1989, 2 (03) :250-269
[4]   DELAYED HYPERSENSITIVITY-TYPE GRANULOMA FORMATION AND DERMAL REACTION INDUCED AND ELICITED BY A SOLUBLE FACTOR ISOLATED FROM SCHISTOSOMA MANSONI EGGS [J].
BOROS, DL ;
WARREN, KS .
JOURNAL OF EXPERIMENTAL MEDICINE, 1970, 132 (03) :488-+
[5]  
BOROS DL, 1975, J IMMUNOL, V114, P1437
[6]  
CHENSUE SW, 1981, J IMMUNOL, V127, P363
[7]  
COLLEY DG, 1975, J IMMUNOL, V115, P150
[8]   ADOPTIVE SUPPRESSION OF GRANULOMA-FORMATION BY LYMPHOCYTES-T AND BY LYMPHOID-CELLS SENSITIVE TO CYCLOPHOSPHAMIDE [J].
COLLEY, DG ;
LEWIS, FA ;
TODD, CW .
CELLULAR IMMUNOLOGY, 1979, 46 (01) :192-200
[9]  
DALTON JP, 1986, J IMMUNOL, V136, P4689
[10]  
DAMIAN RT, 1984, CONTEMP TOP IMMUNOBI, V12, P359