PURIFICATION AND BIOCHEMICAL-PROPERTIES OF CALMODULIN IN ENTAMOEBA-HISTOLYTICA AND ITS DISTRIBUTION DURING SECRETION OF ELECTRON-DENSE GRANULES

被引：25

作者：

MUNOZ, MD

OSHEAALVAREZ, MD

PEREZGARCIA, J

WEINBACH, EC

MORENO, MA

DELATORRE, M

MAGOS, MA

TOVAR, R

机构：

[1] HOSP ESPECIALIDADES CTR MED LA RAZA, INST MEXICANO SEGURO SOCIAL, MEXICO CITY 06760, DF, MEXICO

[2] NIAID, PARASIT DIS LAB, BETHESDA, MD 20892 USA

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 1992年 / 103卷 / 03期

关键词：

D O I：

10.1016/0305-0491(92)90363-V

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1. Calmodulin (CaM) was detected during secretion of electron-dense granules by Entamoeba histolytica trophozoites with immunofluorescence. 2. It was purified to apparent homogeneity by chromatography with a yield of 2.26 mug of calmodulin/mg of protozoan protein. Purity was established by gel electrophoresis. 3. The parasite calmodulin has properties characteristic of calmodulin isolated from other eukaryotes: an apparent molecular weight of 19 or 17 kDa in presence of EGTA or CaCl2, respectively, activation in a calcium dependent manner of bovine heart cyclic nucleotide phosphodiesterase, and its UV spectrum.

引用

页码：517 / 521

页数：5

共 28 条

[1] ISOLATION AND CHARACTERIZATION OF A CALMODULIN LACKING TRIMETHYLLYSINE FROM THE FAT-BODY OF THE SILKWORM, BOMBYX-MORI [J].