A NOVEL EX-VIVO METHOD FOR ASSAYING ADHESION OF CANCER-CELLS TO THE PERITONEUM

被引：23

作者：

ASAO, T

YAZAWA, S

KUDO, S

TAKENOSHITA, S

NAGAMACHI, Y

机构：

[1] GUNMA UNIV,SCH MED,DEPT LEGAL MED,MAEBASHI,GUMMA 371,JAPAN

[2] GUNMA UNIV,SCH MED,DEPT ANAT,MAEBASHI,GUMMA 371,JAPAN

来源：

CANCER LETTERS | 1994年 / 78卷 / 1-3期

关键词：

PERITONEUM; CELL ADHESION; PERITONEAL DISSEMINATION;

D O I：

10.1016/0304-3835(94)90031-0

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

A novel ex vivo method to determine the cell adhesion of cancer cells to the peritoneum was described. The wells of a microtiter plate were filled with cell suspension and sealed using mouse peritoneum. The peritoneum was fixed using a plastic cover and the plate was turned upside down and incubated for cell adhesion. After incubation for 80 min, the plate was centrifuged and non-adherent cells were assayed by MTT assay. Human cancer cells (MKN28, MKN45, MKN74, KM12C and KM12SM) adhered to the mouse peritoneum as well as cells from mouse (Colon26) and the ratio of cells attached to the peritoneum was estimated to be between 12.8 and 66.4%. This simple method could be useful to investigate the adhesion molecule associated with peritoneal dissemination.

引用

页码：57 / 62

页数：6

共 18 条

[1]

ARCHER S, 1990, SURGERY, V108, P502

[2]

ASAO T, 1991, CANCER, V68, P44, DOI 10.1002/1097-0142(19910701)68:1<44::AID-CNCR2820680109>3.0.CO

[3]

2-J

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