MODULATION OF OXIDATIVE STRESS BY BETA-CAROTENE IN CHICKEN-EMBRYO FIBROBLASTS

The ability of beta-carotene to protect against oxidative stress in vitro was assessed. Primary cultures of chicken embryo fibroblasts (CEF) were oxidatively stressed by exposure to paraquat (PQ). Activities of the antioxidant enzymes superoxide dismutase (SOD; EC 1.15.1.1), catalase (CAT; EC 1.11.1.6) and glutathione peroxidase (GSH-Px; EC 1.11.1.9) were measured as indices of oxidative stress, CEF incubated with 0 . 25 mM-PQ for 18 h exhibited increased SOD and CAT activities and decreased GSH-Px activity compared with the control (P < 0 . 001). Incorporation of added beta-carotene (0 . 1 mu M) into 0 . 25 mM-PQ-treated CEF returned SOD activity to that seen in non-PQ-treated cells, beta-Carotene (0 . 1 mu M) reduced the CAT activity from that seen in PQ-treated cells and returned the GSH-Px activity to its central value thus protecting the cells against PQ-induced oxidative stress. However, at higher concentrations of beta-carotene (10 mu M), SOD and CAT activities increased significantly (P < 0 . 001) relative to non-PQ-treated cells and GSH-Px activity decreased relative to its control value. Similar trends were observed when CEF grown in beta-carotene-enriched media (01-10 mu M) were oxidatively stressed by exposure to 0 . 25 mM-PQ for 18 h.