PRODUCTION OF AN EXTRACELLULAR CHITINOLYTIC SYSTEM BY TALAROMYCES-EMERSONII CBS-814.70

被引：10

作者：

HENDY, L ^{[1
]}

GALLAGHER, J ^{[1
]}

WINTERS, A ^{[1
]}

HACKETT, TJ ^{[1
]}

MCHALE, L ^{[1
]}

MCHALE, AP ^{[1
]}

机构：

[1] UNIV DUBLIN TRINITY COLL, MOYNE INST, DEPT MICROBIOL, DUBLIN 2, IRELAND

来源：

BIOTECHNOLOGY LETTERS | 1990年 / 12卷 / 09期

关键词：

D O I：

10.1007/BF01088192

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The thermophilic fungus Talaromyces emersonii CBS 814.70 produces a thermostable extracellular chitinolytic system when cultured on chitin containing media. The chitinolytic system consists of chitinase (EC 3.2.1.14) and N-acetylglucosaminidase (EC 3.2.1.30). Using fluorescent substrate analogues, in zymogram staining of polyacrylamide gradient and isoelectric focusing gels on which the chitinase system was electrophoresed and focused, respectively, it was found that a number of bands could be resolved. Using isoelectric focusing it was observed that at least 4 extracellular forms of chitinase activity are produced. © 1990 Kluwer Academic Publishers.

引用

页码：673 / 678

页数：6

共 13 条

[1]

BERGER LR, 1958, BIOCHIM BIOPHYS ACTA, V29, P522

[2] PRODUCTION, ISOLATION AND PARTIAL CHARACTERIZATION OF AN AMYLASE SYSTEM PRODUCED BY TALAROMYCES-EMERSONII CBS-814.70 [J].

BUNNI, L ;

MCHALE, L ;

MCHALE, AP .

ENZYME AND MICROBIAL TECHNOLOGY, 1989, 11 (06) :370-375

[3] CELLULASE COMPLEX IN CULTURE FILTRATE OF THERMOPHILIC FUNGUS, TALAROMYCES-EMERSONII [J].

FOLAN, MA ;

COUGHLAN, MP .

INTERNATIONAL JOURNAL OF BIOCHEMISTRY, 1978, 9 (10) :717-722

[4]

Jeuniaux C., 1966, METHOD ENZYMOL, V8, P644, DOI DOI 10.1016/0076-6879(66)08117-5

[5]

KNORR D, 1986, BIOCHEMISTRY-US, V155, P644

[6]

MCHALE A, 1982, J GEN MICROBIOL, V128, P2327

[7] THE CELLULOLYTIC SYSTEM OF TALAROMYCES-EMERSONII - PURIFICATION AND CHARACTERIZATION OF THE EXTRACELLULAR AND INTRACELLULAR BETA-GLUCOSIDASES [J].

MCHALE, A ;

COUGHLAN, MP .

BIOCHIMICA ET BIOPHYSICA ACTA, 1981, 662 (01) :152-159

[8]

MCHALE AP, 1986, ENZ MICROBIOL TECHNO, V9, P749

[9]

MCHALE AP, UNPUB

[10] A STUDY OF METHODS FOR PREPARING OLIGOSACCHARIDES FROM CELLULOSE [J].

MILLER, GL ;

DEAN, J ;

BLUM, R .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1960, 91 (01) :21-26

← 1 2 →