ISOLATION AND CHARACTERIZATION OF THE CDNA FOR PEA CHLOROPLAST SECA EVOLUTIONARY CONSERVATION OF THE BACTERIAL-TYPE SECA-DEPENDENT PROTEIN-TRANSPORT WITHIN CHLOROPLASTS

被引：38

作者：

NOHARA, T ^{[1
]}

NAKAI, M ^{[1
]}

GOTO, A ^{[1
]}

ENDO, T ^{[1
]}

机构：

[1] NAGOYA UNIV,FAC SCI,DEPT CHEM,NAGOYA,AICHI 46401,JAPAN

来源：

FEBS LETTERS | 1995年 / 364卷 / 03期

关键词：

SECA; CHLOROPLAST; PROTEIN IMPORT; THYLAKOID;

D O I：

10.1016/0014-5793(95)00415-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report here the isolation of the cDNA for pea chloroplast SecA. Pea SecA encodes a polypeptide of 1,011 amino acids and shows high sequence similarity with cyanobacterial SecA, Pea SecA was synthesized as a larger precursor and was imported into isolated chloroplasts in vitro, The purified pea SecA, which was expressed in Escherichia coli cells, stimulated the in vitro import of the 33 kDa protein of the oxygen-evolving complex into thylakoids, These results indicate that higher plant chloroplasts contain a bacterial-type SecA protein-dependent system for the intraorganellar protein transport into thylakoids.

引用

页码：305 / 308

页数：4

共 24 条

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