DYNAMIC LIGHT-SCATTERING STUDY OF THE EFFECT OF MG2+ AND ATP ON SYNTHETIC MYOSIN-FILAMENTS

The dynamic light scattering (DLS) method provides us with information about the apparent diffusion coefficient, D-app, as well as the static scattering intensity, I-s, of particles in solution. For long but thin rods with length L and diameter d, the dependence on L and dof D-app, is quite different from that of I-s. By means of DLS we studied synthetic myosin filaments of rabbit skeletal muscle in solution at pH 8.3 and 10 degrees C. It appeared that Mg2+ ions induced thickening and lengthening of the filaments, whereas ATP (and ADP) induced thinning and shortening (depolymerization) of the filaments. When ATP was added to the filament preparation in the presence of Mg2+ ions, it was clearly observed that thinning of the filament (or splitting into subfilaments) occurred before shortening (or depolymerization).