MONOMER-TRIMER TRANSITION OF HUMAN PLACENTA ESTRADIOL DEHYDROGENASE

被引：16

作者：

HAGERMAN, DD

机构：

[1] Department of Biochemistry, Department of Obstetrics and Gynecology, University of Colorado School of Medicine, Denver

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 1969年 / 134卷 / 01期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/0003-9861(69)90266-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Human placenta estradiol dehydrogenase (17β-hydroxysteroid:NAD(P)-oxidoreductase; EC 1.1.1.51) prepared with precautions to prevent subtle denaturation exhibits a concentration-dependent monomer-trimer transition. The kinetic properties of the enzyme show that the monomeric form of the protein can catalyze both net steroid dehydrogenation and substrate-mediated transhydrogenation. In contrast, the trimer catalyzes only substrate-mediated transhydrogenation. The trimerization is demonstrable by analysis of the kinetics, by gel permeation chromatography, and by density gradient ultracentrifugation. © 1969.

引用

页码：196 / &

共 20 条

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