IN VITRO INTERACTION OF 1-ANILINO 8-NAPHTALENE SULFONATE WITH EXCITABLE MEMBRANES ISOLATED FROM ELECTRIC ORGAN OF ELECTROPHORUS ELECTRICUS

被引：52

作者：

KASAI, M

CHANGEUX, JP

MONNERIE, L

机构：

[1] Département de Biologie Moléculaire, Institut Pasteur, Paris

[2] Laboratoire de Chimie Macromoléculaire, Ecole de Physique et Chimie, Paris

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1969年 / 36卷 / 03期

关键词：

D O I：

10.1016/0006-291X(69)90581-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In vitro binding of 1-anilino 8-naphtalene sulfonate (ANS) to membrane fragments derived from the innervated, excitable, surface of electroplax cells is accompanied by a strong enhancement of fluorescence intensity and polarization. Effects of temperature on ANS binding indicate that a structural reorganization of the membrane fragments occurs between 30 and 50°C. Fluorescence polarization of bound ANS is insensitive to changes of solvent viscosity upon addition of sucrose. Ca++ and d-tubocurarine modifies ANS binding. © 1969.

引用

页码：420 / &

共 12 条

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