SITE-SPECIFIC MUTAGENESIS OF HUMAN CHORIONIC-GONADOTROPIN (HCG)-BETA SUBUNIT - INFLUENCE OF MUTATION ON HCG PRODUCTION

被引：23

作者：

AZUMA, C ^{[1
]}

MIYAI, K ^{[1
]}

SAJI, F ^{[1
]}

KAMIURA, S ^{[1
]}

TOKUGAWA, Y ^{[1
]}

KIMURA, T ^{[1
]}

OHASHI, K ^{[1
]}

KOYAMA, M ^{[1
]}

IIJIMA, Y ^{[1
]}

KASHIWAI, T ^{[1
]}

HAYASHIZAKI, Y ^{[1
]}

TANIZAWA, O ^{[1
]}

机构：

[1] OSAKA UNIV,DEPT LAB MED,OSAKA 553,JAPAN

来源：

JOURNAL OF MOLECULAR ENDOCRINOLOGY | 1990年 / 5卷 / 02期

关键词：

D O I：

10.1677/jme.0.0050097

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

The heterodimeric glycoprotein hormones, human chorionic gonadotrophin (hCG), LH, TSH and FSH, consist of two non-covalently linked subunits, the α and β subunits. The β subunit is specific for each hormone and is responsible for the biological specificity, but the β subunit of different hormones show some degree of structural homology. The CAGY (cysteine-alanine-glycine-tyrosine) region is one of the amino acid sequences that is homologous in different β subunits and is highly conserved between species. In the present study, site-specific in-vitro mutagenesis was used to change three individual nucleotides in the centre of the CAGY region of the hCG-β subunit, and the effects of these mutations on hCG production was determined by in-vitro transcription and then translation in Xenopus laevis oocytes. The results indicate that the CAGY region, particularly the glycine residue at position 36 in the β subunit, is essential for the production of hCG. This finding is consistent with previous studies showing that this region is necessary for the biological activity of human TSH.

引用

页码：97 / 102

页数：6

共 14 条

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