SIMULTANEOUS DETERMINATION OF VITAMIN-A ACETATE, VITAMIN-D2, AND VITAMIN-E ACETATE IN MULTIVITAMIN MINERAL TABLETS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH COUPLED COLUMNS

A reverse phase high performance liquid chromatographic method for the simultaneous determination of vitamin A acetate (retinol acetate), vitamin D2 (ergocalciferol) and vitamin E acetate (d,/-α-tocopherol acetate) in multivitamin mineral tablets has been developed. The method requires dissolution of the sample In water-ethanol-pyridlne solution (50:46:4), extraction of the vitamins into warm hexane, addition of cholesterol benzoate internal standard and separation with a methanol-water gradient elution on coupled µBondapak Phenyl-µBondapak C18 columns. Detection of the vitamins and internal standard is monitored at 280 nm with separation accomplished In approximately 50 min. The assay is specific for each vitamin and typical relative standard deviations for analysis of dosage forms are 0.059, 0.065, and 0.023 for vitamin A acetate, vitamin D2, and vitamin E acetate, respectively. © 1979, American Chemical Society. All rights reserved.