QUANTITATION OF GLYCOSAMINOGLYCAN HEXOSAMINE USING 3-METHYL-2-BENZOTHIAZOLONE HYDRAZONE HYDROCHLORIDE

被引：141

作者：

SMITH, RL

GILKERSON, E

机构：

[1] Stanford University, Orthopedic Research, Stanford, CA 94305

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 98卷 / 02期

关键词：

D O I：

10.1016/0003-2697(79)90170-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This procedure allows analysis of hexosamine present in glycosaminoglycans under conditions of mild acid treatment. It utilizes the color complex formed by interaction of 3-methyl-2-benzothiazolone hydrazone hydrochloride (MBTH) and the 2,5-anhydrohexoses produced by deamination of hexosamines. Both glucosamine and galactosamine behave equivalently in this assay. © 1979.

引用

页码：478 / 480

页数：3

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