HIGH-ACCURACY MOLECULAR MASS DETERMINATION OF PROTEINS USING MATRIX-ASSISTED LASER DESORPTION MASS-SPECTROMETRY

被引:355
作者
BEAVIS, RC
CHAIT, BT
机构
[1] Rockefeller University, New York
关键词
D O I
10.1021/ac00216a020
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A method for obtaining protein molecular masses with an accuracy of approximately ±0.01 % by matrix-assisted laser desorption using an internal calibrant is described. The technique allows accurate mass determinations of protein sample sizes as small as 1 pmol. High concentrations of organic and inorganic contaminants (e.g. 1 M urea) do not strongly affect either the signal Intensity or the mass assignment. The ability to assign an accurate molecular mass to a protein is contingent on the observation of clearly resolved protonated molecule ions in the mass spectrum. © 1990, American Chemical Society. All rights reserved.
引用
收藏
页码:1836 / 1840
页数:5
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