ICP4, THE MAJOR TRANSCRIPTIONAL REGULATORY PROTEIN OF HERPES-SIMPLEX VIRUS TYPE-1, FORMS A TRIPARTITE COMPLEX WITH TATA-BINDING PROTEIN AND TFIIB

被引:152
作者
SMITH, CA
BATES, P
RIVERGONZALEZ, R
GU, BH
DELUCA, NA
机构
[1] UNIV PITTSBURGH,SCH MED,DEPT MOLEC GENET & BIOCHEM,PITTSBURGH,PA 15261
[2] HARVARD UNIV,SCH MED,DEPT MICROBIOL & MOLEC GENET,BOSTON,MA 02115
关键词
D O I
10.1128/JVI.67.8.4676-4687.1993
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The ICP4 protein of herpes simplex virus can either increase or decrease the rate of transcription mediated by RNA polymerase II, depending on the target promoter. The interplay of DNA-protein and protein-protein contacts determining ICP4 function has yet to be characterized, and consequently the molecular mechanism by which the protein acts remains unclear. ICP4 can transactivate minimal promoters containing only TATA homologies, and therefore it is reasonable to hypothesize that ICP4 works by influencing the TATA-dependent assembly of general transcription factors via specific protein-protein interactions. This study directly addresses this hypothesis by determining whether ICP4 affects the assembly of general transcription factors on templates bearing a TATA box and an ICP4-binding site. Using gel retardation and footprinting assays, we found that ICP4 forms a tripartite complex with TFIIB and either the TATA-binding protein (TBP) or TFIID. The formation of this complex was not the result of simple tripartite occupancy of the DNA but the consequence of protein-protein interactions. In the presence of all three proteins, the affinity of ICP4 and TBP for their respective binding sites was substantially increased. Using mutant derivatives of ICP4 and defective versions of promoters, we also demonstrated that the ability of ICP4 to regulate gene expression correlated with its ability to form a tripartite complex with TFIIB and TBP in vitro.
引用
收藏
页码:4676 / 4687
页数:12
相关论文
共 70 条
[1]   THE PSEUDORABIES IMMEDIATE EARLY PROTEIN STIMULATES INVITRO TRANSCRIPTION BY FACILITATING TFIID - PROMOTER INTERACTIONS [J].
ABMAYR, SM ;
WORKMAN, JL ;
ROEDER, RG .
GENES & DEVELOPMENT, 1988, 2 (05) :542-553
[2]   ADENOVIRUS PROMOTERS AND E1A TRANSACTIVATION [J].
BERK, AJ .
ANNUAL REVIEW OF GENETICS, 1986, 20 :45-79
[3]   5 INTERMEDIATE COMPLEXES IN TRANSCRIPTION INITIATION BY RNA POLYMERASE-II [J].
BURATOWSKI, S ;
HAHN, S ;
GUARENTE, L ;
SHARP, PA .
CELL, 1989, 56 (04) :549-561
[4]   IDENTIFICATION OF HERPES-SIMPLEX VIRUS-DNA SEQUENCES WHICH ENCODE A TRANS-ACTING POLYPEPTIDE RESPONSIBLE FOR STIMULATION OF IMMEDIATE EARLY TRANSCRIPTION [J].
CAMPBELL, MEM ;
PALFREYMAN, JW ;
PRESTON, CM .
JOURNAL OF MOLECULAR BIOLOGY, 1984, 180 (01) :1-19
[5]   A GENETIC APPROACH TO PROMOTER RECOGNITION DURING TRANS INDUCTION OF VIRAL GENE-EXPRESSION [J].
COEN, DM ;
WEINHEIMER, SP ;
MCKNIGHT, SL .
SCIENCE, 1986, 234 (4772) :53-59
[6]   ISOLATION AND CHARACTERIZATION OF A LARGE MOLECULAR-WEIGHT POLYPEPTIDE OF HERPES-SIMPLEX VIRUS TYPE-1 [J].
COURTNEY, RJ ;
BENYESHM.M .
VIROLOGY, 1974, 62 (02) :539-551
[7]   ATP ENHANCES THE BINDING OF SIMIAN VIRUS-40 LARGE T-ANTIGEN TO THE ORIGIN OF REPLICATION [J].
DEB, SP ;
TEGTMEYER, P .
JOURNAL OF VIROLOGY, 1987, 61 (12) :3649-3654
[8]   ACTIVATION OF IMMEDIATE-EARLY, EARLY, AND LATE PROMOTERS BY TEMPERATURE-SENSITIVE AND WILD-TYPE FORMS OF HERPES-SIMPLEX VIRUS TYPE-1 PROTEIN ICP4 [J].
DELUCA, NA ;
SCHAFFER, PA .
MOLECULAR AND CELLULAR BIOLOGY, 1985, 5 (08) :1997-2008
[9]   PHYSICAL AND FUNCTIONAL DOMAINS OF THE HERPES-SIMPLEX VIRUS TRANSCRIPTIONAL REGULATORY PROTEIN-ICP4 [J].
DELUCA, NA ;
SCHAFFER, PA .
JOURNAL OF VIROLOGY, 1988, 62 (03) :732-743
[10]   ACTIVITIES OF HERPES-SIMPLEX VIRUS TYPE-1 (HSV-1) ICP4 GENES SPECIFYING NONSENSE PEPTIDES [J].
DELUCA, NA ;
SCHAFFER, PA .
NUCLEIC ACIDS RESEARCH, 1987, 15 (11) :4491-4511