EFFECT OF PROPIONATE ON IN-VIVO CARBOHYDRATE-METABOLISM IN STREPTOZOCIN-INDUCED DIABETIC RATS

被引:11
作者
CAMERONSMITH, D [1 ]
COLLIER, GR [1 ]
ODEA, K [1 ]
机构
[1] DEAKIN UNIV, SCH NUTR & PUBL HLTH, DEPT HUMAN NUTR, GEELONG, VIC 3217, AUSTRALIA
来源
METABOLISM-CLINICAL AND EXPERIMENTAL | 1994年 / 43卷 / 06期
关键词
D O I
10.1016/0026-0495(94)90122-8
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Undigested carbohydrates and some dietary fibers are fermented in the large intestine to form short-chain fatty acids (SCFA), including acetate, propionate, and butyrate. It has been suggested that some of the beneficial effects of high-carbohydrate, high-fiber diets on carbohydrate and lipid metabolism are mediated by the metabolism of SCFA in the liver. Propionate has been shown in vitro to decrease glucose production in rat hepatocytes. The aim of the present study was to investigate the effects of propionate on carbohydrate metabolism in normal and streptozocin (STZ)-induced diabetic male Sprague-Dawley rats. Rats were fed a high-fat diet with or without sodium propionate supplementation (either 0.5% or 5% wt/wt) for 4 weeks. At the completion of the feeding period, body weight and liver glycogen concentrations were significantly decreased in STZ-diabetic rats and were unaffected by propionate supplementation. Although STZ-diabetic animals had elevated fasting plasma glucose, cholesterol, and triglyceride levels relative to nondiabetic rats, propionate supplementation had no significant effect on these parameters in either group. Basal and insulin-stimulated carbohydrate metabolism were assessed using the euglycemic clamp technique in overnight-fasted animals with 3(H)-6-glucose infusion. As expected, basal hepatic glucose production (HGP) was higher and the metabolic clearance rate of glucose (MCR) was lower in STZ-diabetic rats. High-dose insulin infusion (3 mU · kg-1 · min-1) suppressed HGP in nondiabetic and diabetic animals and increased the MCR in nondiabetic animals. However, propionate supplementation did not alter basal or insulin-stimulated HGP or the MCR in either nondiabetic or diabetic animals. It is concluded that oral propionate supplementation had no detectable effect on carbohydrate or lipid metabolism in either nondiabetic or STZ-diabetic rats. © 1994.
引用
收藏
页码:728 / 734
页数:7
相关论文
共 55 条
[1]   ACETATE TOLERANCE AND THE KINETICS OF ACETATE UTILIZATION IN DIABETIC AND NONDIABETIC SUBJECTS [J].
AKANJI, AO ;
HOCKADAY, TDR .
AMERICAN JOURNAL OF CLINICAL NUTRITION, 1990, 51 (01) :112-118
[2]  
ANDERSON JW, 1984, P SOC EXP BIOL MED, V177, P372
[3]  
BEUERS U, 1990, BIOCHEM INT, V21, P405
[4]  
BLAIR JB, 1973, J BIOL CHEM, V248, P3608
[5]   INVIVO INSULIN RESISTANCE IN STREPTOZOTOCIN-DIABETIC RATS - EVIDENCE FOR REVERSAL FOLLOWING ORAL VANADATE TREATMENT [J].
BLONDEL, O ;
BAILBE, D ;
PORTHA, B .
DIABETOLOGIA, 1989, 32 (03) :185-190
[6]  
BUGUAT M, 1987, COMP BIOCH PHYSL B, V86, P439
[7]   STUDY OF MECHANISM OF INHIBITION OF KETOGENESIS BY PROPIONATE IN BOVINE LIVER [J].
BUSH, RS ;
MILLIGAN, LP .
CANADIAN JOURNAL OF ANIMAL SCIENCE, 1971, 51 (01) :121-&
[8]  
CHAN TM, 1976, P SOC EXP BIOL MED, V151, P372
[9]   EFFECT OF PROPIONATE ON METABOLISM OF PYRUVATE AND LACTATE IN PERFUSED RAT-LIVER [J].
CHAN, TM ;
FREEDLAND, RA .
BIOCHEMICAL JOURNAL, 1972, 127 (03) :539-+
[10]  
CHEN WJL, 1984, P SOC EXP BIOL MED, V175, P215, DOI 10.3181/00379727-175-41791