CALORIMETRIC CHARACTERIZATION OF THE STABLE COMPLEX OF MYOSIN SUBFRAGMENT 1 WITH ADP AND BERYLLIUM FLUORIDE

被引：30

作者：

BOBKOV, AA

KHVOROV, NV

GOLITSINA, NL

LEVITSKY, DI

机构：

[1] A N BACH BIOCHEM INST,MOSCOW 117071,RUSSIA

[2] MOSCOW MV LOMONOSOV STATE UNIV,A N BELOZERSKY INST PHYS CHEM BIOL,MOSCOW 119899,RUSSIA

来源：

FEBS LETTERS | 1993年 / 332卷 / 1-2期

关键词：

MYOSIN SUBFRAGMENT 1; BERYLLIUM FLUORIDE; SCANNING MICROCALORIMETRY; RABBIT SKELETAL MUSCLE;

D O I：

10.1016/0014-5793(93)80485-D

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The thermal unfolding of the myosin subfragment 1 (S1) in its stable complex with ADP and beryllium fluoride (S1 . ADP . BeF3-) was studied by differential scanning calorimetry. It has been shown that the structure of the S1 molecule in the S1 . ADP . BeF3- complex is similar to that of S1 in its complex with ADP and orthovanadate (S1 . ADP . V(i)) but differs radically from that of nucleotide-free S1 and S1 in the S1 . ADP complex. It is concluded that the S1 . ADP . BeF3- complex can be considered, like the S1 . ADP . V(i) complex, a stable structural analogue of the myosin head . ADP . P(i) transition state of the myosin-catalyzed ATP hydrolysis.

引用

页码：64 / 66

页数：3

共 14 条

[1] CHARACTERIZATION OF MYOSIN-PRODUCT COMPLEXES AND OF PRODUCT-RELEASE STEPS DURING MAGNESIUM ION-DEPENDENT ADENOSINE-TRIPHOSPHATASE REACTION [J].