IN-VIVO FUNCTIONAL-RELATIONSHIPS AMONG TERMINAL PROTEINS OF BACILLUS-SUBTILIS PHI-29-RELATED PHAGES

被引：8

作者：

BRAVO, A ^{[1
]}

HERMOSO, JM ^{[1
]}

SALAS, M ^{[1
]}

机构：

[1] UNIV AUTONOMA MADRID,CTR BIOL MOLEC SEVERO OCHOA,CSIC,UAM,E-28049 MADRID,SPAIN

来源：

GENE | 1994年 / 148卷 / 01期

关键词：

EXPRESSION SYSTEM; TRANSCOMPLEMENTATION; PHAGE NF; PZA; M2; PROTEIN-PRIMED DNA REPLICATION;

D O I：

10.1016/0378-1119(94)90242-9

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Gene 3 of the Bacillus subtilis phage phi 29 encodes the terminal protein (TP), which acts as a primer in the initiation of viral DNA replication. We have developed an in vivo functional assay for the phi 29 TP based on the ability of TP-producing B. subtilis non-suppressor (su(-)) cells to support DNA replication of a phi 29 sus3 mutant phage. This trans-complementation assay has been used to study in vivo functional relationships between the TP of phi 29 and related phages. Our results demonstrate that phi 29 TP functionally substitutes the TP of phage PZA, whereas replication of phage Nf DNA cannot take place in vivo using the phi 29 TP as a primer.

引用

页码：107 / 112

页数：6

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