THE ROLE OF PH OF THE MOBILE-PHASE IN ION-INTERACTION RP-HPLC

被引：23

作者：

GENNARO, MC

GIACOSA, D

ABRIGO, C

机构：

[1] Dipartimento di Chimica Ancditica, Universitd di Torino, 10125, Torino, Via P. Giuria

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY | 1994年 / 17卷 / 20期

关键词：

D O I：

10.1080/10826079408013623

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The dependence of retention on pH of the mobile phase is investigated in reversed-phase ion interaction chromatography, being the mobile phase an aqueous solution of 5.0 mM octylammonium and ortho-phosphoric acid at different pH values. The analytes considered were amines, diamines, amides, and species characterized by different functionalities. The behaviour of retention as a function of pH is discussed with comparison with literature data and correlation with pk(a) values is shown. The optimization of separation and resolution in a mixture of acids and amines through pH variations is also presented.

引用

收藏

页码：4365 / 4380

页数：16

相关论文

共 19 条

[1] EXTENSION OF THE ELECTROSTATIC RETENTION MODEL OF REVERSED-PHASE ION-PAIR HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY TO INCLUDE THE EFFECT OF THE ELUENT PH [J].

BARTHA, A ;

STAHLBERG, J ;

SZOKOLI, F .

JOURNAL OF CHROMATOGRAPHY, 1991, 552 (1-2) :13-22

[2] SEPARATION OF IONIC COMPOUNDS BY REVERSED-PHASE LIQUID-CHROMATOGRAPHY - AN UPDATE OF ION-PAIRING TECHNIQUES [J].

BIDLINGMEYER, BA .

JOURNAL OF CHROMATOGRAPHIC SCIENCE, 1980, 18 (10) :525-539

[3] SIMULTANEOUS-OPTIMIZATION OF PH AND ORGANIC MODIFIER CONTENT OF THE MOBILE PHASE FOR THE SEPARATION OF CHLOROPHENOLS USING A DOEHLERT DESIGN [J].

BOURGUIGNON, B ;

MARCENAC, F ;

KELLER, HR ;

DEAGUIAR, PF ;

MASSART, DL .

JOURNAL OF CHROMATOGRAPHY, 1993, 628 (02) :171-189

[4] APPLICATION OF A MIXTURE DESIGN TECHNIQUE TO THE SIMULTANEOUS-OPTIMIZATION OF ANALYSIS TIME AND RESOLUTION IN REVERSED-PHASE ION-PAIR LIQUID-CHROMATOGRAPHY USING A MINIMAL RESOLUTION PLOT [J].

COENEGRACHT, PMJ ;

VANTUYEN, N ;

METTING, HJ ;

COENEGRACHTLAMERS, PJM .

JOURNAL OF CHROMATOGRAPHY, 1987, 389 (02) :351-367

[5] OPTIMIZATION OF REVERSE-PHASE LIQUID-CHROMATOGRAPHIC SEPARATION OF WEAK ORGANIC-ACIDS [J].

DEMING, SN ;

TUROFF, MLH .

ANALYTICAL CHEMISTRY, 1978, 50 (04) :546-548

[6]

DERHOUWEN OAG, 1981, J CHROMATOGR, V209, P393

[7] ROLE OF THE ALKYL CHAIN-LENGTH OF THE ION INTERACTION REAGENT, FLOW-RATE, COLUMN PACKING AND DETECTION IN ION INTERACTION REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY IN SEPARATIONS OF ANIONS USING AMINE SALICYLATES [J].

GENNARO, MC .

JOURNAL OF CHROMATOGRAPHY, 1988, 449 (01) :103-113

[8]

GENNARO MC, 1993, J LIQ CHROMATOGR, V16, P2751

[9]

GENNARO MC, IN PRESS J LIQ CHROM

[10]

HEARN MTW, 1979, J CHROMATOGR, V185, P429, DOI 10.1016/S0021-9673(00)85619-8