HETEROLOGOUS EXPRESSION OF CDNAS ENCODING BARLEY (HORDEUM-VULGARE) (1-]3)-BETA-GLUCANASE ISOENZYME GV

被引：11

作者：

XU, PL ^{[1
]}

HARVEY, AJ ^{[1
]}

FINCHER, GB ^{[1
]}

机构：

[1] UNIV ADELAIDE,DEPT PLANT SCI,GLEN OSMOND,SA 5064,AUSTRALIA

来源：

FEBS LETTERS | 1994年 / 348卷 / 02期

基金：

澳大利亚研究理事会;

关键词：

ALTERNATIVE POLYADENYLATION SITE; BACTERIAL EXPRESSION; BARLEY; GENE FAMILY; (1-]3)-BETA-GLUCANASE ISOENZYME GV;

D O I：

10.1016/0014-5793(94)00610-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two cDNAs have been isolated from libraries generated from poly(A)(+)RNA of young barley roots and leaves, using a cDNA encoding barley (1 --> 3)-beta-glucanase isoenzyme GII as a probe. Nucleotide sequence analyses and ribonuclease protection assays show that the two cDNAs differ only in the length of their 3'-untranslated regions; the corresponding mRNAs are likely to originate from a single gene by tissue-specific processing at separate polyadenylation sites. When the coding region of the cDNA is expressed in E. coli, the resultant protein catalyses the hydrolysis of (1 --> 3)-beta-glucan with an action pattern characteristic of a (1 --> 3)-beta-glucan endohydrolase (EC 3.2.1.39). The enzyme has been designated isoenzyme GV of the barley (1 --> 3)-beta-glucanase family.

引用

页码：206 / 210

页数：5

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