SEXUAL-RESPONSE OF SACCHAROMYCES-CEREVISIAE - PHOSPHORYLATION OF YEAST GLYOXALASE-I BY A CELL EXTRACT OF MATING FACTOR TREATED-CELLS

被引:20
作者
INOUE, Y
CHOI, BY
MURATA, K
KIMURA, A
机构
[1] Research Institute for Food Science, Kyoto University, Uji
[2] Research and Development Center, Miwon Co., Ltd., Dobong-ku, Seoul
关键词
D O I
10.1093/oxfordjournals.jbchem.a123159
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The phosphorylation of glyoxalase I was observed when the phosphatase-treated enzyme was incubated in the presence of [γ--32P] ATP and a cell extract prepared from α-type yeast cells which had been treated with the culture supernatant of a-type yeast cells. The phosphorylated protein was identified as glyoxalase I by using anti-glyoxalase I rabbit immunoglobulin G. © 1990 Copyright, 1990 by the Journal of Biochemistry.
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页码:4 / 6
页数:3
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