SEQUENCE-ANALYSIS OF AMPLIFIED T(14-18) CHROMOSOMAL BREAKPOINTS IN B-CELL LYMPHOMAS

被引：12

作者：

EICK, S ^{[1
]}

KRIEGER, G ^{[1
]}

BOLZ, I ^{[1
]}

KNEBA, M ^{[1
]}

机构：

[1] UNIV GOTTINGEN,ZENTRUM CHIM FIS,HAMATOL ONKOL ABT,ROBERT KOCH STR 40,W-3400 GOTTINGEN,GERMANY

来源：

JOURNAL OF PATHOLOGY | 1990年 / 162卷 / 02期

关键词：

asymmetric PCR; bcl‐2; chromosomal breakpoint; DNA sequence analysis; lymphoma; N‐region; PCR;

D O I：

10.1002/path.1711620205

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

We have explored different strategies for sequencing of major breakpoint (mbr) junctional regions in t(14;18) chromosomal transiocations—the most frequent chromosomal abnormality observed in B‐cell lymphomas. We demonstrate that coupling of the preparation of single‐stranded DNA by asymmetric polymerase chain reaction (PCR) and direct sequencing is the method of choice for the rapid and precise determination of clone‐specific bcl‐2/JH fusion gene sequences. The rapidity, relative ease, and accuracy of the technique, described for the nucleotide sequence analysis of mbr t(14;18) breakpoints, permits the analysis of a relatively large number of samples and should be considered as part of the clinical evaluation of lymphoma patients. Furthermore, by providing sequence information of clone‐specific DNA regions, the procedure should reduce the risk of false‐positive results from PCR. Copyright © 1990 John Wiley & Sons, Ltd.

引用

页码：127 / 133

页数：7

共 33 条

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