IMPROVED SCREENING-TEST FOR ABNORMAL-HEMOGLOBINS FROM DRIED BLOOD-SAMPLES

被引：10

作者：

ALTLAND, K ^{[1
]}

KAEMPFER, M ^{[1
]}

GRANDA, H ^{[1
]}

机构：

[1] UNIV LAHABANA,DEPT MED GENET,LAHABANA,CUBA

来源：

HUMAN GENETICS | 1979年 / 53卷 / 01期

关键词：

D O I：

10.1007/BF00289457

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A method is described wherin blood samples taken from adults or newborns and dried on filter paper can be used for hemoglobin analysis within 2 years after sampling. The samples are eluted in 8 M urea in the presence of 5% 2-mercaptoethanol and 2% of the neutral detergent Nonidet P-40. Then the individual α, β, γ, and δ chains are separated by means of electrofocusing in 8 M urea-PAA gels. Up to 96 samples can be applied to a gel using multiple syringes. Several hundred samples can be analyzed daily by one person. This method may be especially useful for preventive programs against sickle cell anemia as well as for human mutation monitoring systems. © 1979 Springer-Verlag.

引用

页码：97 / 100

页数：4

共 5 条

[1] ALTLAND K, 1977, ELECTROFOCUSING ISOT, P295
[2] ABNORMAL HUMAN HAEMOGLOBINS - SEPARATION AND CHARACTERIZATION OF ALPHA AND BETA CHAINS BY CHROMATOGRAPHY AND DETERMINATION OF 2 NEW VARIANTS HB CHESAPEAKE AND HB J (BANGKOK)
CLEGG, JB
NAUGHTON, MA
WEATHERA.DJ
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1966, 19 (01) : 91 - &
[3] ECONOMIC HIGH-SPEED ELECTROPHORETIC SCREENING SYSTEM FOR HEMOGLOBIN-S AND OTHER PROTEINS
HEREDERO, L
GRANDA, H
SUAREZAG.JA
ALTLAND, K
[J]. HUMANGENETIK, 1974, 21 (02): : 167 - 177
[4] HEREDERO L, 1976, CLIN CHIM ACTA, V71, P515
[5] DETECTION OF SICKLE GENE IN HUMAN FETUS - POTENTIAL FOR INTRAUTERINE DIAGNOSIS OF SICKLE-CELL ANEMIA
KAN, YW
DOZY, AM
FRIGOLETTO, FD
ALTER, BP
NATHAN, DG
[J]. NEW ENGLAND JOURNAL OF MEDICINE, 1972, 287 (01) : 1 - +

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