CHARACTERIZATION OF RNA-MEDIATED RESISTANCE TO TOMATO SPOTTED WILT VIRUS IN TRANSGENIC TOBACCO PLANTS

被引：132

作者：

DEHAAN, P ^{[1
]}

GIELEN, JJL ^{[1
]}

PRINS, M ^{[1
]}

WIJKAMP, IG ^{[1
]}

VANSCHEPEN, A ^{[1
]}

PETERS, D ^{[1
]}

VANGRINSVEN, MQJM ^{[1
]}

GOLDBACH, R ^{[1
]}

机构：

[1] AGR UNIV WAGENINGEN,DEPT VIROL,6700 EM WAGENINGEN,NETHERLANDS

来源：

BIO-TECHNOLOGY | 1992年 / 10卷 / 10期

关键词：

D O I：

10.1038/nbt1092-1133

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Recently high levels of protection against tomato spotted wilt virus (TSWV), a negative-strand RNA virus infecting plants, have been obtained by transforming tobacco with viral nucleoprotein (N) gene sequences. Here we demonstrate that this protection is primarily due to the presence of N gene transcripts in the cells of transgenic plants, and hence appears to be RNA-mediated. Further, transgenic tobacco plants are only protected to isolates and strains of TSWV and not to other tospoviruses that share considerable nucleotide sequence homology in their N genes to TSWV. In addition to being protected after mechanical inoculation, the transgenic tobacco plants are also resistant to inoculation using viruliferous thrips, i.e. Frankliniella occidentalis (Perg.), one of the most important natural vector species.

引用

页码：1133 / 1137

页数：5

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