DETERMINATION OF FLAVONOIDS BY MICELLAR ELECTROKINETIC CAPILLARY CHROMATOGRAPHY

HPCE based on cetyltrimethylammonium bromide (CTAB) or cholate micellar electrokinetic capillary chromatography (MECC) was found suitable for the separation of individual flavonoid glycosides following a rapid and simple technique of isolation, purification and group separation. Kaempferol and quercetin glycosides with varying degrees of glycosylation, and with or without additional esterification on the carbohydrate part, were included in the study. The influence of temperature and voltage as well as electrolyte, CTAB and organic modifier concentrations in the buffer on the migration order, migration times, and peak areas of the flavonoids was investigated. The method developed gives possibilities for the separation and specific determination of flavonoids isolated from vegetative parts of cruciferous plants.