PRECOLUMN DERIVATIZATION OF BIOGENIC-AMINES AND AMINO-ACIDS WITH 9-FLUORENYLMETHYL CHLOROFORMATE AND HEPTYLAMINE

被引：54

作者：

KIRSCHBAUM, J ^{[1
]}

LUCKAS, B ^{[1
]}

BEINERT, WD ^{[1
]}

机构：

[1] FA E MERCK,D-64293 DARMSTADT,GERMANY

来源：

JOURNAL OF CHROMATOGRAPHY A | 1994年 / 661卷 / 1-2期

关键词：

D O I：

10.1016/0021-9673(93)E1107-B

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid and fully automated pre-column derivatization method for the determination of primary and secondary biogenic amines and amino acids is described. The derivatization reagent 9-fluorenylmethyl chloroformate is used, together with heptylamine for removing the excess of reagent. The derivatization procedure is described in detail. Chromatographic separation and detection of biogenic amines only or of biogenic amines and amino acids is possible. Repeatability data and applications of the technique are presented.

引用

页码：193 / 199

页数：7

共 21 条

[1] ALVAREZCOQUE MCG, 1989, ANAL BIOCHEM, V178, P1
[2] Askar A, 1986, BIOGENE AMINE LEBENS
[3] NAPHTHALENE-2,3-DICARBOXALDEHYDE CYANIDE ION - A RATIONALLY DESIGNED FLUOROGENIC REAGENT FOR PRIMARY AMINES
DEMONTIGNY, P
STOBAUGH, JF
GIVENS, RS
CARLSON, RG
SRINIVASACHAR, K
STERNSON, LA
HIGUCHI, T
[J]. ANALYTICAL CHEMISTRY, 1987, 59 (08) : 1096 - 1101
[4] HIGH-RESOLUTION REVERSED-PHASE LIQUID-CHROMATOGRAPHY SYSTEM FOR THE ANALYSIS OF COMPLEX SOLUTIONS OF PRIMARY AND SECONDARY AMINO-ACIDS
EINARSSON, S
FOLESTAD, S
JOSEFSSON, B
LAGERKVIST, S
[J]. ANALYTICAL CHEMISTRY, 1986, 58 (08) : 1638 - 1643
[5] FULLY AUTOMATED AMINO-ACID-ANALYSIS FOR PROTEIN AND PEPTIDE HYDROLYSATES BY PRECOLUMN DERIVATIZATION WITH 9-FLUORENYL METHYLCHLOROFORMATE AND 1-AMINOADAMANTANE
GUSTAVSSON, B
BETNER, I
[J]. JOURNAL OF CHROMATOGRAPHY, 1990, 507 : 67 - 77
[6] HUI JY, 1983, J ASSOC OFF ANA CHEM, V66, P853
[7] STABILITY OF ORTHO-PHTHALALDEHYDE-DERIVED ISOINDOLES
JACOBS, WA
LEBURG, MW
MADAJ, EJ
[J]. ANALYTICAL BIOCHEMISTRY, 1986, 156 (02) : 334 - 340
[8] JOOSTEN HMLJ, 1987, NETH MILK DAIRY J, V41, P247
[9] KARMAS E, 1981, LEBENSM WISS TECHNOL, V14, P273
[10] KIRSCHBAUM J, 1993, UNPUB VORSAULENDERIV

← 1 2 3 →