T-CELL EPITOPE ANALYSIS WITH PEPTIDES SIMULTANEOUSLY SYNTHESIZED ON CELLULOSE MEMBRANES - FINE MAPPING OF 2 DQ DEPENDENT EPITOPES

被引：22

作者：

ADLER, S

FRANK, R

LANZAVECCHIA, A

WEISS, S

机构：

[1] GBF,D-38124 BRAUNSCHWEIG,GERMANY

[2] BASEL INST IMMUNOL,CH-4005 BASEL,SWITZERLAND

来源：

FEBS LETTERS | 1994年 / 352卷 / 02期

关键词：

T CELL; EPITOPE; MHC CLASS II; PEPTIDE SYNTHESIS;

D O I：

10.1016/0014-5793(94)00950-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Several MHC class II restricted, CD4(+) human T cell clones from three donors were induced with soluble matrix protein of influenza virus. The epitopes recognized by these clones were mapped using a complete set of overlapping 15-mer peptides synthesized with the newly developed SPOT method which allows the simple simultaneous synthesis of numerous peptides on cellulose membranes. Fine analysis of two clones by truncation of the stimulatory peptide by single subsequent amino acids from either the NH2- or the COOH-terminus revealed the minimal stimulatory determinants of two DQ dependent T cell epitopes.

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页码：167 / 170

页数：4

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