A MODIFIED COLORIMETRIC MICRO METHOD FOR LONG-CHAIN FATTY ACIDS AND ITS APPLICATION FOR ASSAY OF LIPOLYTIC ENZYMES

被引:76
作者
MAHADEVAN, S
DILLARD, CJ
TAPPEL, AL
机构
[1] Department of Food Science and Technology, University of California, Davis
关键词
D O I
10.1016/0003-2697(69)90052-9
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A modification of the Duncombe method for measurement of free fatty acids is presented. Substitution of diphenylcarbohydrazide for diethyldithiocarbamate color complexing agent increases the sensitivity of the method 4- to 6-fold. The absorbancy of the color produced is measured at 540 mμ. Applications of the method for the assay of pancreatic lipase, and rat liver lysosomal lipase and phospholipase are described. © 1969.
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页码:387 / +
页数:1
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