ISOLATION AND CHARACTERIZATION OF LEVANSUCRASE-ENCODING GENE FROM BACILLUS-AMYLOLIQUEFACIENS

被引：45

作者：

TANG, LB ^{[1
]}

LENSTRA, R ^{[1
]}

BORCHERT, TV ^{[1
]}

NAGARAJAN, V ^{[1
]}

机构：

[1] DUPONT CO,DIV CENT RES & DEV,E228 RM 310C,POB 80228,WILMINGTON,DE 19880

来源：

GENE | 1990年 / 96卷 / 01期

关键词：

Phagemid; recombinant DNA; signal sequence; sucrose regulation;

D O I：

10.1016/0378-1119(90)90345-R

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The gene encoding levansucrase (LVS) from Bacillus amyloliquefaciens (sacB[BamP]) was isolated, sequenced and expressed in Bacillus subtilis. Analysis of the nucleotide sequence of socB[BamP] reveals extensive homology with that of the B. subtilis LVS-encoding gene in the promoter and coding region. The sacB[BamP] gene cloned in a multicopy plasmid is induced by sucrose in B. subtilis. © 1990.

引用

页码：89 / 93

页数：5

共 18 条

[1] PROTEIN-PHOSPHORYLATION REGULATES TRANSCRIPTION OF THE BETA-GLUCOSIDE UTILIZATION OPERON IN ESCHERICHIA-COLI [J].

AMSTERCHODER, O ;

HOUMAN, F ;

WRIGHT, A .

CELL, 1989, 58 (05) :847-855

[2]

AYERMICH S, 1987, MOL GEN GENET, V208, P114

[3] INDUCTION OF LEVANSUCRASE IN BACILLUS-SUBTILIS - AN ANTITERMINATION MECHANISM NEGATIVELY CONTROLLED BY THE PHOSPHOTRANSFERASE SYSTEM [J].

CRUTZ, AM ;

STEINMETZ, M ;

AYMERICH, S ;

RICHTER, R ;

LECOQ, D .

JOURNAL OF BACTERIOLOGY, 1990, 172 (02) :1043-1050

[4] CLONING OF THE SACS GENE ENCODING A POSITIVE REGULATOR OF THE SUCROSE REGULON IN BACILLUS-SUBTILIS [J].

DEBARBOUILLE, M ;

KUNST, F ;

KLIER, A ;

RAPOPORT, G .

FEMS MICROBIOLOGY LETTERS, 1987, 41 (02) :137-140

[5]

Dedonder R, 1966, METHOD ENZYMOL, V8, P500

[6]

HENNER DJ, 1988, GENETICS BIOTECHNOLO, V2, P3

[7]

LEPESANT JA, 1976, MICROBIOLOGY 1976, P58

[8] POSITIVE AND NEGATIVE REGULATION OF THE BGL OPERON IN ESCHERICHIA-COLI [J].

MAHADEVAN, S ;

REYNOLDS, AE ;

WRIGHT, A .

JOURNAL OF BACTERIOLOGY, 1987, 169 (06) :2570-2578

[9]

Maniatis T., 1982, MOL CLONING

[10]

MCLAUGHLIN JR, 1981, J BIOL CHEM, V256, P1283

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