ENZYMATIC METHOD FOR DETERMINATION OF THE DEGREE OF DEACETYLATION OF CHITOSAN

被引：41

作者：

NANJO, F

KATSUMI, R

SAKAI, K

机构：

[1] Research and Development Laboratories, Yaizu Suisan Kagaku Industry Co., Ltd., Yaizu, Shizuoka, 425

来源：

ANALYTICAL BIOCHEMISTRY | 1991年 / 193卷 / 02期

关键词：

D O I：

10.1016/0003-2697(91)90002-B

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new method was developed for the accurate determination of the degree of deacetylation of chitosan. The method involves the complete hydrolysis of chitosan to glucosamine and N-acetylglucosamine by a cooperative action of chitosanolytic enzymes exo-β-d-glucosaminidase, β-N-acetylhexosaminidase, and chitosanase, and subsequent determination of the monosaccharides by specific colorimetric assays or HPLC. The conditions required for the complete hydrolysis of chitosan were examined and the degree of deacetylation of several chitosan samples was determined. © 1991.

引用

页码：164 / 167

页数：4

共 20 条

[1] STUDIES ON CHITOSAN .1. DETERMINATION OF THE DEGREE OF N-ACETYLATION OF CHITOSAN BY ULTRAVIOLET SPECTROPHOTOMETRY AND GEL-PERMEATION CHROMATOGRAPHY [J].